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Paper 68

ACT January 2008

Form 61B · Science Test · 40 questions

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Paper 68 · ACT January 2008 · Form 61B · Science Test

2 mm) was the cofactor in all trials, and the temperature was varied from trial to trial (see Table 2). 2+ Enzymes calalyze (speed up) the rate of a biological reaction without being consumed in the reaction. Pyrococ- cus furiosu.f, an organism that lives in ocean thermal vents where temperatures remain near IOO°C, produLes the enzyme prolidase. Prolidase catalyzes the breakdown of proteins to release the amino acid proline. An enzyme's activiry is a measure of its effectiveness for a given reac- tion expressed as a percent of its maximum measured effectiveness. Table 2 Temperature Activity (°c) (%) 40 50 60 70 80 90 100 22 42 60 94 100 Experiment I Many enzymes function only in the presence of a metal ion, called a cofacfor. One ML of a standard solution containing prolidase and 0.4 mm (millimolar. a unit of concentration) cobalt ion (Co ') was mixed with I ML of a solution of Proteill X (of which proline is a component) at a pH of 7.0 and l OO°C. After 10 min. a substance that react.% with free proline to produce a bright red color was added to the solution. The solution was diluted to l 00 ML. and the intensity of the red color was measured and used to calculate the activity. The procedure was repeated for dif- 2+ Experiment 3 Experiment 2 was repeated, but from trial to trial, temperature was held constant at l OO°C, and pH was varied (see Table 3). ferent concentrations of Co and then for manganese ion (Mn ') and zinc ion (Zn ") at all of the concentrations (see Table l). Table 3 Table I Actlvity (%) pH Ion concentration Activity (%) (mm) 2+ 41 59 92 100 94 24 16 62 86 100 92 84 35 49 68 66 57 ACT-61B-SAMPLE GO ON TO THE NEXT PAGE. 40000000004 l. If, in Experiment 2, a trial had been done at 85°C. tbe activity would most likely have been:

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